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Antibodies sure to the blocking peptide not bind for the epitope around the concentrate on protein. By comparing the staining from your blocked antibody as opposed to the antibody by yourself, you can see which staining is certain.
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This guideline outlines additional doable optimization of mobile and immunoassay parameters to ensure the best possible results are...
This tutorial outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind particularly into the goal antibody and block antibody binding. These peptide usually is made up of the epitope recognized because of the antibody.
Goal Information and facts Cyclin E belongs for the really conserved cyclin household, whose users are characterized by a remarkable periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation styles which contribute to the temporal coordination of each mitotic function. Cyclin E varieties a complex with and capabilities as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates with the G1-S period boundary which is degraded as cells progress by way of S stage.
Overexpression of this gene is observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two extra splice variants were being documented but detailed nucleotide sequence data is not nevertheless available.
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